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In this project we will exploit the genetic tractability and imaging potential of the zebrafish model to address the following questions: i) What is the role of heparan sulfate in shaping interstitial chemokine gradients during infection in vivo?To address this question, we will exploit CRISPR-Cas9 gene targeting to generate knock-in transgenic zebrafish, whereby an endogenous chemokine (Cxcl8) that is normally produced at sites of infection is replaced by a fluorescently tagged, and thereby tractable, version of the same chemokine."The Niqoyastcdje—'Place-where-they-came-up,'" said Brouillard, elucidating for her.

However it may be, we will for the present leave to others the task of elucidating the question of origin. Whewell's work was written for the purpose of elucidating them, which he does at great length.

Site-selective introduction of fluorine into polyketides and other natural products is a particularly interesting area of exploration given the demonstrated effectiveness of fluorine in modulating the behavior of small-molecule therapeutics.

Here, we show that fluorine can be inserted site-selectively by an engineered polyketide synthase system via a fluorinated monomer that becomes covalently tethered to the enzyme to complete a canonical reaction cycle.

Hereafter I hope to elucidate at length these phenomena of expression.

It may help to elucidate this matter if we regard it from another point of view.




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